| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Brachyspira murdochii | TGCGAAGTTACAACGGCAG | TACCCTAGCCTGCCAAAGC | 58.77 | 59.39 | 234 | 105 |
100.00%
|
100.00%
|
| Primer 22 | Brachyspira murdochii | GTCATCAGGCTGCCGTAAG | CCCCTTCTCTTGAAGTTCTGC | 58.32 | 58.57 | 168 | 104 |
100.00%
|
100.00%
|
| Primer 23 | Brachyspira murdochii | GGTCATCAGGCTGCCGTAAG | CCCCTTCTCTTGAAGTTCTGC | 60.81 | 58.57 | 169 | 104 |
100.00%
|
100.00%
|
| Primer 24 | Brachyspira murdochii | CAGTGCCTTCAGTTGATTTGC | CGGCATCAAACTTCCGCTG | 58.33 | 59.86 | 290 | 104 |
100.00%
|
100.00%
|
| Primer 25 | Brachyspira murdochii | GGAAGCAGACGCAGATATGC | TAAGCTCGCGTGCTACTGG | 59.14 | 59.86 | 241 | 104 |
100.00%
|
100.00%
|
| Primer 26 | Brachyspira murdochii | GGAAGCAGACGCAGATATGC | TGTCTCTAAGCTCGCGTGC | 59.14 | 60.15 | 247 | 104 |
100.00%
|
100.00%
|
| Primer 27 | Brachyspira murdochii | GCAAAACAATGCCGTTCATGC | GGCAGTGTATTTGGTGCTGG | 60.14 | 59.47 | 194 | 104 |
100.00%
|
100.00%
|
| Primer 28 | Brachyspira murdochii | TGCCATTGGAGTTGGAGCTG | TCATCTCCGGTGATGCGTG | 60.61 | 59.86 | 213 | 102 |
100.00%
|
100.00%
|
| Primer 29 | Brachyspira murdochii | ACCGTTTCTTGCTTGGGC | ACACGACCGCACAAAGCA | 58.56 | 60.51 | 158 | 102 |
100.00%
|
100.00%
|
| Primer 30 | Brachyspira murdochii | ACCGTTTCTTGCTTGGGC | ACAGCAAACACGACCGCA | 58.56 | 60.51 | 165 | 102 |
100.00%
|
100.00%
|